Signac is an extension of Seurat for the analysis, interpretation, and exploration of single-cell chromatin datasets.
Signac is currently focused on the analysis of single-cell ATAC-seq data, but new features will be added as experimental methods for measuring other chromatin-based modalities at single-cell resolution become more widespread. Signac supports the following features:
Please see the Signac vignettes page for examples.
To use Signac first make sure Bioconductor is installed:
# Install bioconductor if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install()
install.packages("Signac")
install.packages("devtools") devtools::install_github("timoast/signac", ref = "develop")
For information about installing Seurat, see the Seurat website
It can also be useful (but not essential) to install species-specific packages from Bioconductor: